| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 100mg | 电议 |
Cell lines | Primary calvaria osteoblasts in mice |
Preparation Method | Osteoblasts were incubated with varying concentrations of Genistein and different assays viz. cell proliferation, differentiation, calcium deposition, cell cycle progression, antioxidant ability, and osteogenic gene expression were performed. |
Reaction Conditions | 1、5 and 10 μM Genistein for 9 days |
Applications | Results revealed that Genistein markedly induced cell growth and osteoblast differentiation depending upon dose. Antioxidant ability of Genistein, which reduced the H2O2- induced intracellular oxidative stress in osteoblasts. Genistein treatment upregulated the expression of osteoblastic genes of Runt-related transcription factor 2 (Runx2), bone morphogenetic proteins 2 (BMP2), and osteocalcin. |
Animal models | Adult male Wistar rats |
Preparation Method | Eight groups of adult male Wistar rats, fed with either standard chow diet or high-fat diet, were treated with BPA (50μg/kg/day), genistein (10mg/kg/day), and BPA plus genistein for 35 weeks, respectively. Metabolic parameters in serum and liver were determined; the hematoxylin/eosin and oil Red O staining were used to observe liver histologically |
Dosage form | 10mg/kg/day genistein for 35 weeks |
Applications | No difference of body weight gain, total energy intake, liver weight/body weight or body fat percentage in both STD- and HFD-fed sub-groups was observed after treatment with BPA, genistein, or BPA plus genistein. Genistein alleviated lipid metabolism disorder and decreased the mRNA and protein expression of PPARγ, and increased the protein expression of LC3II in liver of HFD-fed rats. |
| 产品描述 | Genistein is an isoflavone belonging to the flavonoid group of compounds and is found in a number of plants. It's a tyrosine kinase inhibitor. Results revealed that Genistein markedly induced cell growth and osteoblast differentiation depending upon dose. Antioxidant ability of GS, which reduced the H2O2- induced intracellular oxidative stress in osteoblasts. GS treatment upregulated the expression of osteoblastic genes of Runt-related transcription factor 2 (Runx2), bone morphogenetic proteins 2 (BMP2), and osteocalcin[7]. Pre-treatment with genistein did not affect insulin-induced tyrosine kinase activity of the insulin receptor or activation of protein kinase B. On the other hand, genistein acted as a direct inhibitor of insulin-induced glucose uptake in 3T3-L1 adipocytes with an IC(50) of 20 microM[4]. Migration of inflammatory cells is also inhibited by genistein through diminishing adherence of leukocytes to endothelial cells[5].Genistein decreases NF-kappa B DNA binding and abrogates NF-kappa B activation by DNA-damaging agents, H2O2 and tumor necrosis factor-alpha, in prostate cancer cells regardless of androgen sensitivity. Genistein reduces phosphorylation of the inhibitory protein I kappa B alpha and blocks the nuclear translocation of NF-kappa B, prohibiting DNA binding and preventing NF-kappa B activation[6]. Genistein decreases NF-kappa B DNA binding and abrogates NF-kappa B activation by DNA-damaging agents, H2O2 and tumor necrosis factor-alpha, in prostate cancer cells regardless of androgen sensitivity. Genistein reduces phosphorylation of the inhibitory protein I kappa B alpha and blocks the nuclear translocation of NF-kappa B, prohibiting DNA binding and preventing NF-kappa B activation[1]. No difference of body weight gain, total energy intake, liver weight/body weight or body fat percentage in both STD- and HFD-fed sub-groups was observed after treatment with BPA, genistein, or BPA plus genistein. Genistein alleviated lipid metabolism disorder and decreased the mRNA and protein expression of PPARγ, and increased the protein expression of LC3II in liver of HFD-fed rats[2]. Cultivation of the tiny clusters of cells scattered throughout the pancreas, also known as pancreatic islets, were isolated from three to five day-old animals (rats) with genistein, resulting in a rapid and more enhanced insulin exudation in a dose-dependent manner, genistein substantially increased insulin secretion in pancreatic islets of adult mice as well[3]. References: |
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