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2,3-Diaminonaphthalene
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
2,3-Diaminonaphthalene图片
包装与价格:
包装价格(元)
10mM (in 1mL DMSO)电议
100mg电议

产品介绍
2,3-Diaminonaphthalene是一种高选择性的比色和荧光试剂,可用于硒检测,也可用于亚硝酸盐的荧光测定。

Kinase experiment:

Various concentrations of each drug are incubated for 60 min at 37℃ in OS-mL reaction volumes containing PBS (pH 7.4), 200 μM 2,3-Diaminonaphthalene (DAN) and 40 μM Sp/NO. Spermine/NO will spontaneously decompose at pH7.4 to produce 2 mol of NO and 1 mol of spermine with a half-life of 39 min at 37℃. Following the incubation period, 2.5 mL of 10 mM NaOH is added to each tube to stop the reaction. The NO-dependent N-nitrosation of 2,3-Diaminonaphthalene to yield its highly fluorescent N-nitrosated derivative, 2,3-naphthotriazole, is quantified by measuring the fluorescence of each sample using an excitation wavelength of 375 nm and an emission wavelength of 450nm. The concentration of Triazole is determined using standards of the pure 2,3-naphthotriazole[3].

产品描述

2,3 Diaminonaphthalene is a highly selective colorimetric and fluorometric reagent for selenium detection and also used for the fluorometric determination of nitrite.

Poly(2,3-diaminonaphthalene) microspheres as a novel quencher for fluorescence-enhanced nucleic acid detection[1]. 2,3-Diaminonaphthalene (DAN) is possibly a carcinogenic reagent[2].

[1]. Tian J, et al. Poly(2,3-diaminonaphthalene) microspheres as a novel quencher for fluorescence-enhanced nucleic acid detection. Analyst. 2011 Jun 7;136(11):2221-4. [2]. Martínez-Tomé MJ, et al. Immobilization and characterization of 2,3-diaminonaphthalene/cyclodextrin complexes in a sol-gel matrix: a new fluorimetric sensor for nitrite. J Fluoresc. 2009 Jan;19(1):119-25. [3]. Grisham MB, et al. Effects of aminosalicylates and immunosuppressive agents on nitric oxide-dependent N-nitrosation reactions. Biochem Pharmacol. 1994 May 18;47(10):1897-902.