5-Bromouridine is a brominated analog of the nucleoside uridine .¹It can be incorporated into RNA and subsequently detected by antibodies against bromodeoxyuridine .²5-Bromouridine decreases the viability of HL-60 and MOLT-4 cells (LC₅₀s = 10 and 20 μM, respectively). It induces apoptosis and halts the cell cycle at the S phase in HL-60 cells. It is photoreactive, and UV irradiation has been used to cross-link RNA containing 5-bromouridine to proteins in the study of of RNA-protein interactions.^1,35-Bromouridine can also be incorporated into RNA using 5-bromouridine 5'-triphosphate .

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