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Enteropeptidase Fluorogenic Substrate(trifluoroacetate salt)
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Enteropeptidase Fluorogenic Substrate(trifluoroacetate salt)图片
包装与价格：
包装价格(元)
500 µg电议
1mg电议
5mg电议

产品介绍
A fluorogenic substrate of enteropeptidase
Canonical SMILESNCCCC[C@H](NC([C@H](CC(O)=O)NC([C@H](CC(O)=O)NC([C@H](CC(O)=O)NC([C@H](CC(O)=O)NC(C)=O)=O)=O)=O)=O)C(NC1=CC=C(C(C(F)(F)F)=CC(O2)=O)C2=C1)=O.OC(C(F)(F)F)=O
分子式C34H40F3N7O16·XCF3COOH
分子量859.7
溶解度DMF: 30 mg/mL,DMSO: 30 mg/mL,Ethanol: 10 mg/mL,PBS (pH 7.2): 2.5 mg/mL
储存条件-20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Enteropeptidase fluorogenic substrate is a substrate for enteropeptidase that contains a 7-amino-4-trifluoromethylcoumarin (AFC) moiety. Enteropeptidase is a serine protease expressed in the proximal small intestine of higher animals that converts inactive trypsinogen to active trypsin by endoproteolytic cleavage.1,2Enteropeptidase recognizes the highly specific amino acid sequence DDDDK on the fluorogenic substrate and cleaves after the lysine residue, releasing the AFC moiety. Enteropeptidase activity is quantified by fluorescent detection of AFC, which displays excitation/emission spectra of 380/500 nm.3

1.Pepeliaev, S., Krahulec, J., ?ernÝ, Z., et al.High level expression of human enteropeptidase light chain in Pichia pastorisJ. Biotechnol.156(1)67-75(2011) 2.Choi, M.-G., Lee, S., Chung, H.-S., et al.A fluorogenic method for measuring enteropeptidase activity: Spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarinBMB Rep.44(7)458-461(2011) 3.Gossrau, R.Cytochemistry of membrane proteasesHistochem J.17(7)737-771(1985)