Podophyllotoxin-d₆is intended for use as an internal standard for the quantification of podophyllotoxin and picropodophyllotoxin by GC- or LC-MS. Podophyllotoxin is a lignan that has been found inPodophyllumand has diverse biological activities.^1,2,3,4,5It inhibits polymerization of isolated chicken brain tubulin (IC₅₀= 0.6 µM) and induces mitotic arrest at the G₂/M phase in CCRF CEM lymphoblastic leukemia cells when used at concentrations ranging from 0.01 to 5 µg/ml.^2,3Podophyllotoxin (5 µM) is protective against herpes simplex virus 1 (HSV-1) infection in Vero cells.⁴It inhibits the proliferation of several diffuse large B cell lymphoma (DLBCL) cell lines (IC₅₀s = 0.0098-0.017 µM).⁵Podophyllotoxin is a starting material in the semisynthesis of the anticancer compounds etoposide and teniposide .⁶Formulations containing podophyllotoxin have been used in the treatment of external genital warts.

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