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BRD0539
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
BRD0539图片
CAS NO:1403838-79-8
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议

产品介绍
BRD0539 是一种可渗透细胞的,无毒的 CRISPR-Cas9 抑制剂。在体外 DNA 裂解试验中,BRD0539 可抑制化脓性链球菌 Cas9 (SpCas9),IC50 为 22 μM。
Cas No.1403838-79-8
分子式C25H25FN2O3S
分子量452.54
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

BRD0539 is a cell-permeable and non-toxic inhibitor of CRISPR-Cas9. BRD0539 inhibits Streptococcus pyogenes Cas9 (SpCas9) (apparent IC50=22 μM) in an in vitro DNA cleavage assay[1].

BRD0539 dose-dependently blocks the formation of the DNA-bound state in a dose-dependent fashion. BRD0539 impairs the perturbation induced by the 4PAM DNA[1].BRD0539 does not interfere with the SpCas9:gRNA interaction[1].BRD0539 is able to inhibit SpCas9 in the eGFP-disruption assay, BRD0539 is unable to inhibit FnCpf1, a structurally different CRISPR-associated nuclease, in the same assay, further highlighting the specificity of BRD0539[1].

[1]. Basudeb Maji, et al. A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9. Cell. 2019 May 2;177(4):1067-1079.e19.