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5-bromo-3-indolyl-β-D-Galactopyranoside
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
5-bromo-3-indolyl-β-D-Galactopyranoside图片
CAS NO:97753-82-7
包装与价格:
包装价格(元)
100mg电议
500mg电议
1 g电议

产品介绍
5-bromo-3-indolyl-β-D-Galactopyranoside (Bluo-Gal) 是一种显色底物,用于检测细菌 β-D-galactosidase 活性。
Cas No.97753-82-7
别名5-溴-3-吲哚基-beta-D-吡喃半乳糖苷,Bluo-Gal
Canonical SMILESBrC1=CC=C2C(C(O[C@@H]3O[C@@H]([C@@H]([C@@H]([C@H]3O)O)O)CO)=CN2)=C1
分子式C14H16BrNO6
分子量374.2
溶解度DMF: 1 mg/ml,DMSO: 2 mg/ml,Ethanol: insol,PBS (pH 7.2): insol
储存条件-20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

5-Bromo-3-indolyl-β-D-galactopyranoside (Bluo-Gal) is a cell-permeable chromogenic substrate for β-galactosidase.1Upon enzymatic hydrolysis, it generates an indigo-colored precipitate that can be used to measure β-galactosidase activity. Bluo-Gal has been used to detect the expression of β-galactosidase in muscle from transgenic mice expressingE. coli lacZ, the gene that encodes β-galactosidase.

1.Gioglio, L., de Angelis, M.G.C., Boratto, R., et al.An improved method for β-galactosidase activity detection on muscle tissue. A light and electron microscopic studyAnn. Anat.184(2)153-157(2002)