Spermidine-d₆is intended for use as an internal standard for the quantification of spermidine by GC- or LC-MS. Spermidine is an endogenous polyamine.¹It is formed from putrescine by spermidine synthase. Spermidine (25 µM) inhibits the activity of the human inward-rectifying potassium channel K_ir2.3 in a patch-clamp assay.¹It induces autophagy in HeLa cells when used at a concentration of 100 µM and increases the lifespan ofD. melanogaster, yeast, andC. elegans.²Spermidine (30 mM in the drinking water) reduces demyelination of the optic nerve and disease severity in a mouse model of experimental autoimmune encephalomyelitis (EAE).³It reduces increases in blood pressure, left ventricular posterior wall thickness, and heart weight in salt-sensitive Dahl rats fed a high-salt diet, a model of hypertension-induced congestive heart failure.⁴Formulations containing spermidine have been used as dietary supplements.

1.Madeo, F., Eisenberg, T., Pietrocola, F., et al.Spermidine in health and diseaseScience359(6374)eaan2788(2018) 2.Eisenberg, T., Knauer, H., Schauer, A., et al.Induction of autophagy by spermidine promotes longevityNat. Cell Biol.11(11)1305-1314(2009) 3.Guo, X., Harada, C., Namekata, K., et al.Spermidine alleviates severity of murine experimental autoimmune encephalomyelitisInvest. Ophthalmol. Vis. Sci.52(5)2696-2703(2011) 4.Eisenberg, T., Abdellatif, M., Schroeder, S., et al.Cardioprotection and lifespan extension by the natural polyamine spermidineNat. Med.22(12)1428-1438(2016)