PI3K/Akt/mTOR-IN-2 是一种有效的 PI3K/AKT/mTOR 抑制剂。PI3K/Akt/mTOR-IN-2 具有抗癌作用，并对 MDA-MB-231 细胞具有选择性，IC50 为 2.29 μM。PI3K/Akt/mTOR-IN-2 可诱导癌细胞周期阻滞和细胞凋亡 (apoptosis)。
| Cas No. | 2757804-89-8 |
| 分子式 | C17H13F2NO |
| 分子量 | 285.29 |
| 溶解度 | DMSO : ≥ 100 mg/mL (350.52 mM) |
| 储存条件 | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
| Shipping Condition | Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request |
| 产品描述 | PI3K/Akt/mTOR-IN-2 is aPI3K/AKT/mTORpathway inhibitor. PI3K/Akt/mTOR-IN-2 possess anti-cancer effects and selectivity against MDA-MB-231 cells withIC50value of 2.29 μM. PI3K/Akt/mTOR-IN-2 can induce cancer cell cycle arrest andapoptosis[1]. PI3K/Akt/mTOR-IN-2 (compound 23) (0.5 - 100 μM; 72 hours) exhibits effective anti-cancer activity with IC50s of 2.29 - 24.63 μM, of which, IC50in MDA-MB-231 is 2.29 μM[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24 hours) induces growth inhibition of MDA-MB-231 cells by cell cycle arrest at G0/G1[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24, 48 and 72 hours) induces apoptosis in MDA-MB-231 cells with both dose- and time-dependent manners[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 48 hours) increases the expression of Bax, and decreases the expression of Bcl-2 in MDA-MB-231 cells[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24 hours) induces mitochondria-dependent apoptosis in MDA-MB-231 cells through disruption of MMP, accumulation of ROS, depletion of GSH and elevation of intracellular Ca2+[1]. Cell Proliferation Assay | Cell Line: | PC3, BGC-823, A549, MCF-7, MDA-MB-231 cells and MCF-10A cells[1] | | Concentration: | 0.5 - 100 μM | | Incubation Time: | 72 hours | | Result: | Exhibited effective anti-cancer activity with IC50s of 2.29 - 24.63 μM, of which, IC50in MDA-MB-231 was 2.29 μM. |
Cell Cycle Analysis | Cell Line: | MDA-MB-231[1] | | Concentration: | 1 μM, 2 μM and 4 μM | | Incubation Time: | 24 hours | | Result: | Induced growth inhibition of MDA-MB-231 cells by cell cycle arrest at G0/G1. |
Apoptosis Analysis | Cell Line: | MDA-MB-231[1] | | Concentration: | 1 μM, 2 μM and 4 μM | | Incubation Time: | 24, 48 and 72 hours | | Result: | Induced apoptosis in MDA-MB-231 cells with both dose- and time-dependent manners. |
Western Blot Analysis | Cell Line: | MDA-MB-231[1] | | Concentration: | 1 μM, 2 μM and 4 μM | | Incubation Time: | 48 hours | | Result: | Increased the expression of Bax, and decreased the expression of Bcl-2 |
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