A water-soluble and cell-permeable tetrazolium dye
货号:ajcx23964
CAS:150849-52-8
分子式:C19H11IN5O8S2•Na
分子量：651.3
溶解度:DMF: 5 mg/ml,DMSO: 10 mg/ml,PBS (pH 7.2): 10 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

WST-1 is a water-soluble and cell-permeable tetrazolium dye.^1,2,3Upon NADH-dependent enzymatic cleavage by cellular mitochondrial dehydrogenases, formazan is released, which can be quantified by colorimetric detection at 450 nm as a measure of cell viability.

1.Francoeur, A.M., and Assalian, A.Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1Biochemica319-25(1996) 2.Ishiyama, M., Shiga, M., Sasamoto, K., et al.A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dyeChem. Pharm. Bull.41(6)1118-1122(1993) 3.Ishiyama, M., Tominaga, H., Shiga, M., et al.A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violetBiol. Pharm. Bull.19(11)1518-1520(1996)