Background:

C16 Sphingomyelin-d₉is intended for use as an internal standard for the quantification of C16 sphingomyelin by GC- or LC-MS. C16 Sphingomyelin is a naturally occurring sphingolipid.^1,2,3Levels of C16 sphingomyelin are elevated in the prefrontal cortex and cerebellum in a rat model of diabetes induced by streptozotocin , as well as in the liver in mouse models of Niemann-Pick type C1 disease.^1,2Plasma levels of C16 sphingomyelin are decreased in patients with prostate cancer.³Model membranes containing C16 sphingomyelin, 1-palmitoyl-2-oleoyl-sn-glycero-3-PC , and cholesterol have been used in the study of lipid raft dynamics and membrane organization.^4,5
C16 Sphingomyelin-d₉MaxSpec^?standard is a quantitative grade standard of C16 sphingomyelin-d₉(d18:1/16:0-d₉) that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This C16 sphingomyelin-d₉MaxSpec^?standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product.Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

1.Fiedorowicz, A., Prokopiuk, S., Zendzian-Piotrowska, M., et al.Sphingolipid profiles are altered in prefrontal cortex of rats under acute hyperglycemiaNeuroscience256282-291(2014) 2.Praggastis, M., Tortelli, B., Zhang, J., et al.A murine Niemann-Pick C1 I1061T knock-in model recapitulates the pathological features of the most prevalent human disease alleleJ. Neurosci.35(21)8091-8106(2015) 3.Awwad, H.M., Ohlmann, C.H., Stoeckle, M., et al.Choline-phospholipids inter-conversion is altered in elderly patients with prostate cancerBiochimie126108-114(2016) 4.de Almeida, R.F.M., Loura, L.M.S., Fedorov, A., et al.Lipid rafts have different sizes depending on membrane composition: A time-resolved fluorescence resonance energy transfer studyJ. Mol. Biol.346(4)1109-1120(2005) 5.Halling, K.K., Ramstedt, B., NystrÖm, J.H., et al.Cholesterol interactions with fluid-phase phospholipids: Effect on the lateral organization of the bilayerBiophys. J.95(8)3861-3871(2008)