An internal standard for the quantification of linoleoyl ethanolamide
货号:ajcx22832
CAS:1451194-69-6
分子式:C20H33D4NO2
分子量：327.5
溶解度:DMF: 25 mg/ml,DMSO: 25 mg/ml,Ethanol: 50 mg/ml,Ethanol:PBS (pH 7.2)(1:2): 100 μg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

Linoleoyl ethanolamide-d₄contains four deuterium atoms at the hydroxyethyl 1,1',2, and 2' positions. It is intended for use as an internal standard for the quantification of linoleoyl ethanolamide by GC-or LC-mass spectrometry. Linoleoyl ethanolamide is an endocannabinoid detected in porcine brain and murine peritoneal macrophages which contains linoleate in place of the arachidonate moiety of arachidonoyl ethanolamide (AEA).^1,2It has weak affinity for the central cannabinoid (CB₁) and peripheral cannabinoid (CB₂) receptors, exhibiting K_ivalues of 10 µM and 25 µM, respectively.³However, it is only approximately 4-fold less potent than AEA at causing catalepsy in mice (ED₅₀of 26.5 mg/kg).⁴In addition, linoleoyl ethanolamide increases ERK phosphorylation and AP-1-dependent transcription approximately 1.5-fold at 15 µM in a CB-receptor-independent manner.⁵However, cellular toxicity is readily apparent at concentrations of 10-20 µM. Linoleoyl ethanolamide inhibits human fatty acid amide hydrolase-dependent hydrolysis of AEA with a K_ivalue of 9.0 µM, but also is hydrolyzed effectively by the enzyme.^6,7

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