An aminoglycoside antibiotic
货号:ajcx24058
CAS:N/A
分子式:C20H37N3O13•XH2O
分子量：527.5
溶解度:DMSO: 3.3 mg/ml,PBS (pH 7.2): 3.3 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

Hygromycin B is an antibiotic which is used in molecular and cell biology to select for transformed cells expressing the hygromycin resistance gene.^1,2It is an aminoglycoside produced by the bacteriumS. hygroscopicuswhich kills prokaryotic and eukaryotic cells by inhibiting protein synthesis.^3,4The hygromycin resistance gene (hygorhph) encodes a phosphotransferase which inhibits hygromycin through phosphorylation.²

1.Kaster, K.R., Burgett, S.G., Rao, R.N., et al.Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencingNucleic Acids Research11(19)6895-6911(1983) 2.Blochlinger, K., and Diggelmann, H.Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cellsMolecular and Cellular Biology4(12)2929-2931(1984) 3.Eustice, D.C., and Wilhelm, J.M.Mechanisms of action of aminoglycoside antibiotics in eucaryotic protein synthesisAntimicrobial Agents and Chemotherapy26(1)53-60(1984) 4.CabaÑas, M.J., VÁzquez, D., and Modolell, J.Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognitionEuropean Journal of Biochemistry8721-27(1978)