| 包装: | 1mg |
| 规格: | 98% |
| 市场价: | 2227元 |
| 分子量: | 593.71 |
Background:
Fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two colour imaging with Janelia Fluor® 549 SE . Also suitable for flow cytometry. Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19. We recommend that stock solutions of this dye are prepared in anhydrous DMF. To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA. We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Schmidt et al (2016) Live cell imaging reveals the dynamics of telomerase recruitment to telomeres. Cell 166 1188 PMID:27523609 |Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. Elife 5 e17056 PMID:27484239 |Legant et al (2016) High-density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID:26950745 |Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID:25599551 |Deng et al (2015) CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc.Natl.Acad.Sci.USA. 112 11870 PMID:26324940 |Ticau et al (2015) Single-molecule studies of origin licensing reveal mechanisms ensuring bidirectional helicase loading. Cell 161 513 PMID:25892223 |Hong et al (2009) Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome. Proc.Natl.Acad.Sci.U.S.A. 106 14276 PMID:19666497
m.cnreagent.com