used for Click chemistry to label alkyne-modified oligonucleotides
货号:ajcx11144
CAS:N/A
分子式:C36H47N6NaO8S2
分子量：778.91
溶解度:very high in water
纯度：98%
存储：Store at -20°C
库存：现货

Background:

Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions. Sulfo-Cy5 azide is not only a sulfonated but also a popular fluorophore. For labeling proteins and peptides, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.

In mammalian cells, Sulfo-Cy5-azide was incubated with U2OS cells. Finally, the study only find that the cells expressing tRNAIle2-5S fusion RNA can be labeled and imaged with Sulfo-Cy5-azide [1].

参考文献:
[1] Li, F. ; Dong, J.; Hu, X.; Gong, W.; Li, J.; Shen, J.; Tian, H.; Wang, J. A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells. Angewandte Chemie International Edition, 2015, 54(15), 4597–4602.