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EGF Receptor Substrate 2 Phospho-Tyr5
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
规格:98%
分子量:1328.28
包装与价格：
包装价格(元)
1mg电议
5mg电议
10mg电议

产品介绍
EGFReceptorSubstrate2(Phospho-Tyr5)是一种活性肽，它来源于表皮生长因子受体自身磷酸化位点(酪氨酸992)。
货号:ajcx13242
CAS:N/A
分子式:C54H82N13O24
分子量：1328.28
溶解度:Soluble in H2O
纯度：98%
存储：Store at -20°C
库存：现货

Background:

EGF Receptor Substrate 2 (Phospho-Tyr5) is a biologically active peptide derived from an autophosphorylation site (Tyr992) of epidermal growth factor receptor (EGFR).

DADEpYLIPQQG is a generalized PTPase substrate. Both CAV GST-VP2 and TLMV GST-ORF2 are shown to have PTPase activity using both ENDpYINASL and DADEpYLIPQQG. Steady state activities for the reaction of 5 μg of CAV GST-VP2 with ENDpYINASL and DADEpYLIPQQG are 100 and 208%, respectively, of those seen for 2 units of TC-PTP[1]. In the context of DADEpYLIPQQG, the minimal sizes recognized by PTPα are either ADEpYLI or DADEpY-NH2. The kcat/Km value for the parent peptide DADEpYLIPQQG (EGFR988-998) is 1090-fold higher than Tyr(P)[2].


[1]. Peters MA, et al. Chicken anemia virus VP2 is a novel dual specificity protein phosphatase. J Biol Chem. 2002 Oct 18;277(42):39566-73. Epub 2002 Jul 31. [2]. Wu L, et al. Comparative kinetic analysis and substrate specificity of the tandem catalytic domains of the receptor-like protein-tyrosine phosphatase alpha. J Biol Chem. 1997 Mar 14;272(11):6994-7002.

Protocol:

Kinase experiment:

Briefly, the assays are performed in 50 μL volumes in a microtiter plate using the generalized PTPase substrates ENDpYINASL and DADEpYLIPQQG (where pY represents phosphotyrosine) and assay buffer containing 25 mM Tris-HCl (pH 7.4), 50 mM NaCl, 2 mM EDTA, 5 mM dithiothreitol, 0.01% Brij 35, and 1 mg of bovine serum albumin/mL. The reactions are started by the addition of 5 μg of either CAV GST-VP2, GST, CAV GST-VP2 containing the C95S mutation, or TLMV GST-ORF2 or 2 units of the positive control T cell protein-tyrosine phosphatase (TC-PTP) in assay buffer. Control reactions are assayed with either ENDpYINASL substrate alone, DADEpYLIPQQG substrate alone, CAV GST-VP2 without substrate, CAV GST-VP2 containing the C95S mutation without substrate, TLMV GST-ORF2 without substrate, TC-PTP without substrate, or assay buffer with neither enzyme nor substrate. A phosphate standard curve is derived using a supplied phosphate standard. The reactions are terminated by the addition of the malachite green detection reagent.

参考文献:

[1]. Peters MA, et al. Chicken anemia virus VP2 is a novel dual specificity protein phosphatase. J Biol Chem. 2002 Oct 18;277(42):39566-73. Epub 2002 Jul 31.
[2]. Wu L, et al. Comparative kinetic analysis and substrate specificity of the tandem catalytic domains of the receptor-like protein-tyrosine phosphatase alpha. J Biol Chem. 1997 Mar 14;272(11):6994-7002.