WST-8 是一种水溶性四唑鎓染料，与传统的基于 MTS 的测定相比，WST-8 提高了基于 WST-8 的测定的灵敏度。
货号:ajcx11028
CAS:193149-74-5
分子式:C20H14N6NaO11S2+
分子量：600.47
溶解度:DMSO: 1 mg/ml,PBS (pH 7.2): 5 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].

参考文献：
[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.