used for Click chemistry to label alkyne-modified oligonucleotides
货号:ajcx11148
CAS:
分子式:C35H45ClN6O
分子量：601.22
溶解度:≥ 60.1mg/mL in DMSO, ≥ 49.1 mg/mL in EtOH with ultrasonic
纯度：98%
存储：Store at -20°C
库存：现货

Background:

Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Alkyne-modified biomolecules in mixtures of water with organic solvents can be labeled by Cy5 azide.

Cy5/BHQ2, which is widely used in the research, combinations on several TaqMan probes with various degree of stability of secondary structures and provides some advice on the applicability of dye/quencher pairs for DNA dual labeled probes [1].

参考文献:
[1] Farzan, V. M.; Aparin, I.O.; Veselova, O.A.; Podkolzin, A.T.; Shipulin, G.A.; Korshun, V.A.; Zatsepin, T.S. Cy5/BHQ dye-quencher pairs in fluorogenic qPCR probes: effects of charge and hydrophobicity. Analytical Methods, in press.