Background:

Prostaglandin D₂-d₄(PGD₂-d₄) is intended for use as an internal standard for the quantification of PGD₂by GC-or LC-MS. PGD₂is the major eicosanoid product of mast cells and is released in large quantities during allergic and asthmatic anaphylaxis.¹Mastocytosis patients produce excessive amounts of PGD₂, which causes vasodilation, flushing, hypotension, and syncopal episodes.¹PGD₂is also produced in the brainviaan alternative pathway involving a soluble, secreted PGD-synthase also known as β-trace.^2,3In the brain, PGD₂produces normal physiological sleep and lowering of body temperature.^2,3Further pharmacological actions include inhibition of platelet aggregation and relaxation of vascular smooth muscle.⁴PGD₂inhibits human ovarian tumor cell proliferation with an IC₅₀value of 6.8 µM.⁵
PGD₂-d₄MaxSpec^?standard is a quantitative grade standard of PGD₂-d₄that has been prepared specifically for mass spectrometry or any application where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This PGD₂-d₄MaxSpec^?standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product.Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

1.Roberts, L.J., II, and Sweetman, B.J.Metabolic fate of endogenously synthesized prostaglandin D2 in a human female with mastocytosisProstaglandins30(3)383-400(1985) 2.Hayaishi, O.Sleep-wake regulation by prostaglandins D2 and E2J. Biol. Chem.263(29)14593-14596(1988) 3.Onoe, H., Ueno, R., Fujita, I., et al.Prostaglandin D2, a cerebral sleep-inducing substance in monkeysProc. Natl. Acad. Sci. U.S.A.85(11)4082-4086(1988) 4.Giles, H., and Leff, P.The biology and pharmacology of PGD2Prostaglandins35(2)277-300(1988) 5.Kikuchi, Y., Kita, T., Hirata, J., et al.Preclinical studies of antitumor prostaglandins by using human ovarian cancer cellsCancer Metastasis Rev.13(3-4)309-315(1994)