AC-green(VDP-green)是一种β-烯丙基氨基甲酸酯荧光探针，可选择性地对活体系统中的邻位二硫醇蛋白质(VDPs)进行成像(Λex/Λem=400/475nm)。AC-green可以高灵敏度检测降低牛血清白蛋白(rBSA)。AC-green毒性低，灵敏度高，适用于检测活细胞和斑马鱼中的VDP。
货号:ajcx32068
CAS:N/A
分子式:C24H25N3O5
分子量：435.47
溶解度:N/A
纯度：98%
存储：Store at -20°C
库存：现货

Background:

AC-green (VDP-green) is a β-allyl carbamate fluorescent probe for specifically imaging vicinal dithiol proteins (VDPs) in living systems (Λex/Λem=400/475 nm). AC-green can detect the reduced bovine serum albumin (rBSA) with high sensitivity. AC-green displays low toxicity and features high sensitivity, and is suitable for sensing VDPs in living cells and zebrafishes[1].

AC-green (VDP-green) can respond to VDPs with more than 60-fold increase of emission in aqueous solution, while there is no significant interference from biological thiols, amino acids or inorganic salts[1]. AC-green (2 μM; 90 min) has no apparent fluorescence signal within the pH range of 5.0-9.0. Addition of rBSA turns on the fluorescence[1]. AC-gree (10 μM) has low cytotoxicity in HepG2 cells and Hela cells[1]. AC-gree (10 μM; for 15 min) images VDPs in living HepG2 cells and bright green fluorescence appeared. This fluorescence is inhibited when the cells are pretreated with PAO, a popular specificligand for protein vicinal dithiols[1]. AC-gree (10 μM; for 20 min) incubates with zebrafishes has a strong fluorescence signal appeared in the green channel[1].

[1]. Lanning Zhao, et al. A β-allyl carbamate fluorescent probe for vicinal dithiol proteins. Chem Commun (Camb). 2020 Mar 5;56(19):2857-2860.