A neuropeptide with diverse biological activities
货号:ajcx23342
CAS:N/A
分子式:C23H34D4O7
分子量：430.6
溶解度:DMF: 10 mg/ml,DMSO: 10 mg/ml,Ethanol: 10 mg/ml,PBS (pH 7.2): 100 μg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

PGE₂-d₄-1-glyceryl ester contains four deuterium atoms at the 2, 2', 3, and 3' positions. It is intended for use as an internal standard for the quantification of PGE₂-1-glyceryl ester by GC-or LC-mass spectrometry. 2-Arachidonoyl glycerol (2-AG) has been isolated from porcine brain, and has been characterized as the natural endocannabinoid ligand for the CB₁receptor.^1,2Incubation of 2-AG with COX-2 and specific prostaglandin H₂(PGH₂) isomerases in cell cultures and isolated enzyme preparations results in prostaglandin glycerol ester formation.³The biosynthesis of PGH, PGD, PGE, PGF, and TXA-2-glyceryl ester compounds have all been documented. The 2-glyceryl ester moiety equilibrates rapidly (within minutes) with the more stable 1-glyceryl ester, producing a 10:90 2:1-glyceryl ester mixture in typical aqueous media. While the stability and metabolism of these prostaglandin products has been investigated, little is known about their intrinsic biological activity.⁴

1.Sugiura, T., Kodaka, T., Kondo, S., et al.2-Arachidonoylglycerol, a putative endogenous cannabinoid receptor ligand, induces rapid, transient elevation of intracellular free Ca2+ in neuroblastoma X glioma hybrid NG108-15 cellsBiochemical and Biophysical Research Communications22958-64(1996) 2.Sugiura, T., Kodaka, T., Kondo, S., et al.Is the cannabinoid CB1 receptor a 2-arachidonoylglycerol receptor• Structural requirements for triggering a Ca2+ transient in NG108-15 cellsJ. Biochem.122(4)890-895(1997) 3.Kozak, K.R., Crews, B.C., Morrow, J.D., et al.Metabolism of the endocannabinoids, 2-arachidonylgycerol and anandamide, into prostaglandin, thromboxane, and prostacyclin glycerol esters and ethanolamidesJ. Biol. Chem.277(47)44877-44885(2002) 4.Kozak, K.R., Crews, B.C., Ray, J.L., et al.Metabolism of prostaglandin glycerol esters and prostaglandin ethanolamides in vitro and in vivoThe Journal of Biological Chemisty276(40)36993-36998(2001)