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LiCl
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:7447-41-8
包装:100mg
规格:98%
市场价:466元
分子量:42.39

产品介绍
used to precipitate RNA
CAS:7447-41-8
分子式:ClLi
分子量:42.39
纯度:98%
存储:Store at -20°C

Background:

IC50: N/A


LiCl is a typical ionic compound, though the small size of the Li+ ion makes its properties not seen for other alkali metal chlorides, such as the extraordinary solubility in polar solvents and hygroscopic properties.


In vitro: Though precipitation with a monovalent cation or alcohol is much more widely used, LiCl has been frequently used to precipitate RNA. LiCl precipitation gives various advantages over other methods: LiCl does not precipitate DNA, protein and carbohydrate efficiently. LiCl can be also used for removing translation inhibitors or cDNA synthesis from RNA preparations. LiCl can be also used as a simple rapid method for recovering RNA from in-vitro transcription reactions [1]. In addition, a method for isolation of large, translationally active RNA samples has been presented. In this study, the procedure included homogenization of samples in 5 m guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 m LiCl. The advantages of this method include simplicity, speed, avoidance of ultracentrifugation, and its applicability to large numbers of samples. The method yielded large mRNA precursors up to 10 kb [2].


In vivo: Currently, there is no animal data reported.


Clinical trial: Up to now, LiCl has not been used clinically.


参考文献:
[1] BARLOW JJ, MATHIAS AP, WILLIAMSON R, GAMMACK DB.  A simple method for the quantitative isolation of undegraded high molecular weight ribonucleic acid. Biochem Biophys Res Commun.1963 Sep 10;13:61-6.
[2] Cathala, G. , Savouret, J., Mendez, B., West, B. L., Karin, M., Martial, J. A., and Baxter, J. D. (1983). "A Method for Isolation of Intact, Translationally Active Ribonucleic Acid".DNA.2(4): 329–335.