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Docetaxel
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Docetaxel图片
CAS NO:114977-28-5
包装:25mg, 50mg, 100mg
包装与价格:
包装价格(元)
Free Sample (0.5-1 mg)电议
25mg电议
50mg电议
100mg电议

产品名称
Taxotere
产品介绍

生物活性

Docetaxel是一种紫杉醇类似物,通过结合到稳定的微管来抑制微管解聚。Docetaxel是一种细胞毒性剂,尤其对于增殖细胞,Docetaxel促进微管蛋白束的形成,还能诱导持续的有丝分裂阻滞,随后分裂阻滞的细胞凋亡或永久抑制分裂。 Docetaxel抑制微管的动态不稳定性及踏车现象,导致染色体不能分开到子细胞中,这样又会反过来触发有丝分裂过早结束,而不是在细胞周期的特定时期进行抑制作用。 Docetaxel (150 ng/mL) 处理人类小细胞肺癌 N417细胞系 1小时,抑制50% 细胞生长。


化学数据

分子量807.88
分子式C43H53NO14
CAS号114977-28-5
纯度98.36%
溶解性(25°C)DMSO 60 mg/mL
Ethanol 60 mg/mL
储存和运输条件2-8°C, protect from light, dry, sealed
常温运输及临时存放

实验操作 来自于公开的文献,仅供相同实验参考(如实验材料、目的不同,请参考其他文献)

细胞实验
细胞系A549, HCT-116, NCI-H838, KB-3-1, MX-1W, NCI-H1299 and DLD-1 cells
方法Cell Proliferation Assays
Cytotoxicity was assessed by growing cells in the presence of agents for 72 h. Cell survival was measured by the sulforhodamine B (SRB) protein stain method or the ATP-binding assay using the Cell-Titer Glo Luminescent Reporter (GLR) System (Promega, Inc., Madison, WI). The SRB assay was done as previously described (23). For the GLR system, cells were plated robotically at approximately 50% confluency in a 384-well plate and allowed to attach for 12 h at 37°C/5% CO2. After diluting test agents using BioMek 2000 robotic system (Beckman Instruments, Fullerton, CA), agents were added to each well and incubated for 72 h. ATP binding and stabilization of the luminescent signal were performed according to manufacturer's protocol (Promega) following tumor cell lysis. Absorbance was read on a Victor V multilabel plate reader (Perkin-Elmer, Gaithersburg, MD) at a wavelength of A595 and data collected using Wallac 1420 Workstation software.
浓度0~100 nM
处理时间72 h

动物实验
动物模型Athymic nu/nu female mice bearing Lox melanoma cells, KB-3-1 cells and A375SM melanoma cells tumour xenograft model
配制1.4% ethanol/3.5% polysorbate 80 in saline
剂量25 mg/kg
给药处理i.v.

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。


储备液配制

以下数据基于产品分子量,对于特殊产品,请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass1 mg5 mg10 mg
1 mM1.2378 mL6.189 mL12.3781 mL
5 mM0.2476 mL1.2378 mL2.4756 mL
10 mM0.1238 mL0.6189 mL1.2378 mL