Background:

3-O-Acetylbufotalin is a derivate of bufadienolide, with anti-cancer activity[1].

3-O-Acetylbufotalin (2b) inhibits the growth of six human cancer cell lines, with a mean IC50 of 159 nM[1].

[1]. Moreno Y Banuls L, et al. Structure-activity relationship analysis of bufadienolide-induced in vitro growth inhibitory effects on mouse and human cancer cells.