GBF1 inhibitor, potent, reversible and highly specific
CAS：1139889-93-2
分子式：C17H14F2N2
分子量：284.3
纯度：98%
存储：Store at -20°C

Background:

ADP-ribosylation factor 1 (Arf1), when activated by guanine nucleotide exchange factors (ArfGEFs), plays a key role in regulating secretory traffic and membrane transport within the Golgi of eukaryotic cells. Golgicide A is a reversible inhibitor of the brefeldin A-resistant, cis-Golgi ArfGEF, GBF1 (IC50 = 3.3 μM).1 Inhibition of GBF1 via golgicide A can result in rapid dissociation of COPI vesicle coat protein from Golgi membranes and disassembly of the Golgi and trans-Golgi network.[1] Golgicide A does not affect the association of the adaptor protein, AP-1, or the Arf1-binding protein, GCA3, with the trans-Golgi network nor does it interfere with the activity of the ArfGEFs, BIG1 or 2.[1] Golgicide A can arrest the secretion of soluble and membrane-associated proteins at the ER-Golgi intermediate compartment, as well as prevent endocytic transport of Shiga toxin to the trans-Golgi network.[1]

Reference:
[1]. Saenz, J.B., Sun, W.J., Chang, J.W., et al. Golgicide A reveals essential roles for GBF1 in Golgi assembly and function. Nature Chemical Biology 5(3), 157-165 (2009).