您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 产品库 > ID-8
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
ID-8
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
ID-8图片
CAS NO:147591-46-6
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)298.29
FormulaC16H14N2O4
CAS No.147591-46-6
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 60 mg/mL (201.1 mM)
Water: <1 mg/mL
Ethanol: 2 mg/mL (6.7 mM)
Other infoChemical Name: 1-(4-methoxyphenyl)-2-methyl-3-nitro-1H-indol-6-ol
InChi Key: VVZNWYXIOADGSW-UHFFFAOYSA-N
InChi Code: InChI=1S/C16H14N2O4/c1-10-16(18(20)21)14-8-5-12(19)9-15(14)17(10)11-3-6-13(22-2)7-4-11/h3-9,19H,1-2H3
SMILES Code: OC1=CC2=C(C=C1)C([N+]([O-])=O)=C(C)N2C3=CC=C(OC)C=C3
SynonymsID-8; ID 8; ID8
实验参考方法
In Vitro

In vitro activity: ID-8 reversibly maintains the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation. ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs. The mechanism study indicates that ID-8 maintains the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs.


Cell Assay: Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture.

In Vivo
Animal model
Formulation & Dosage
ReferencesBiosci Biotechnol Biochem. 2008 May;72(5):1242-8; Stem Cells Transl Med. 2012 Jan;1(1):18-28.