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CCT007093
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CCT007093图片
CAS NO:176957-55-4
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)272.39
FormulaC15H12OS2
CAS No.176957-55-4
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: <1 mg/mL
Water: <1 mg/mL
DMF: 3 mg/mL (11.0 mM)
Solubility (In vivo)5% DMSO+Corn oil: 1 mg/mL warmed (3.67 mM)
Synonyms CCT-007093; CCT007093; CCT 007093
SMILES Code O=C1/C(CC/C1=C\C2=CC=CS2)=C/C3=CC=CS3
Chemical Name (2E,5E)-2,5-Bis(2-thienylmethylene)-cyclopentanone
实验参考方法
In Vitro

In vitro activity: CCT007093 selectively and potently inhibits human tumor cell lines (MCF-7, KPL-1, and MCF-3B) that overexpress PPM1D. CCT007093 induces cell death via the activation of p38 kinase activity. CCT007093 in combination with paclitaxel, results in synergistic inhibition of the four paclitaxel-resistant triple-negative breast cancer (TNBC) cell lines. CCT007093 selectively promote apoptosis in breast cancer cells and skin transformed keratinocytes that ectopically expressed Wip1, while attenuates the UV-mediated apoptotic response in both skin keratinocytes and a Wip1-null cell model.


Kinase Assay: Recombinant PPM1D (20-50 pmol) is diluted in Tris buffer (50 mM, pH 8), NaCl (100 mM), β-mercaptoethanol (1 mM) or DTT (1 mM) and treated with MnCl2 (0, 1, 10 and 20 mM) or MgCl2 (0 and 40 mM). Where appropriate, inhibitors of PPM1D (10-50 μM) are added and the assay mix incubated for 30 min at room temperature. Recombinant phospho-P38 (200 pmol) is then added and the mixture incubated at 37°C for 1 h. The reaction is quenched by the addition of excess ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulphate-sample loading buffer and boiling for 5 min at 95°C followed by gel electrophoresis and western blotting.


Cell Assay: Cells (MCF-7, KPL-1, and MCF-3B cells) are transfected with a pSUPER plasmid and an additional plasmid expressing the blasticidin resistance gene (pEFBsd) in a molar ratio of 10:1. Cells are plated in 10 cm plates 24 h after transfection. Blasticidin selection (5 μg/ml) is initiated 48 h post-transfection and replenished every 3 days. Colonies are fixed in methanol and stained with crystal violet after 14 days. Colonies are quantified on a Colcount and the surviving fraction (SF) determined.

In Vivo CCT007093 enhances liver regeneration and increases the survival rate of mice after major hepatectomy.
Animal modelMice
Formulation & DosageN/A
ReferencesOncogene. 2008 Feb 14;27(8):1036-44; Breast Cancer Res. 2010;12(3):R41; J Dermatol Sci. 2014 Feb;73(2):125-34.