Dihydroethidium (Hydroethidine;DHE；PD-MY003)

Dihydroethidium(Hydroethidine;PD-MY003)是过氧化物指示剂，在细胞质中具有蓝色荧光，直到被氧化。
CAS：104821-25-2
分子式：C21H21N3
分子量：315.41
纯度：98%
存储：Store at -20°C

Background:

Dihydroethidium is a cell-permeable blue fluorescent dye that upon entering cells interacts with superoxide to form oxyethidium, which intercalates with nucleic acids and emits a red fluorescence detectable qualitatively by fluorescent microscopy or quantitatively by HPLC.¹ It displays excitation/emission spectra of 490/590 nm.² This selective and sensitive probe has been used to detect reactive oxygen species during the phagocytic respiratory burst and for the detection of intracellular superoxide in cultured cells.^3,4,5

参考文献：

1. Cai, H., Dikalov, S., Griendling, K.K., et al. Detection of reactive oxygen species and nitric oxide in vascular cells and tissues: Comparison of sensitivity and specificity. Methods Mol. Med. 139, 293-311 (2007).

2. Laurindo, F.R.M., Fernandes, D.C., and Santos, C.X.C. Assessment of superoxide production and NADPH oxidase activity by HPLC analysis of dihydroethidium oxidation products. Methods Enzymol. 441, 237-260 (2008).

3. Fink, B., Laude, K., McCann, L., et al. Detection of intracellular superoxide formation in endothelial cells and intact tissues using dihydroethidium and an HPLC-based assay. Am. J. Physiol. Cell Physiol. 287, C895-C902 (2004).

4. Robinson, K.M., Janes, M.S., Pehar, M., et al. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc. Natl. Acad. Sci. USA 103(41), 15038-15043 (2006).

5. Salih, H.R., Husfeld, L., and Adam, D. Simultaneous cytofluorometric measurement of phagocytosis, burst production and killing of human phagocytes using Candida albicans and Staphylococcus aureus as target organisms. Clin. Microbiol. Infect. 6(5), 251-258 (2000).