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BAPTA-AM
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
BAPTA-AM图片
CAS NO:126150-97-8
规格:≥98%
包装与价格：
包装价格(元)
1mg电议
2mg电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)764.68
FormulaC34H40N2O18
CAS No.126150-97-8
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 20 mg/mL (26.2 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Other info

Chemical Name: Acetyloxymethyl 2-[N-[2-(acetyloxymethoxy)-2-oxoethyl]-2-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]anilino]acetate

SMILES Code: O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O

Synonyms

BAPTA-AM; BAPTA AM; BAPTA/AM

实验参考方法
In Vitro

In vitro activity: BAPTA-AM, as an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. BAPTA-AM exerts an open channel blocking effect on hERG, hKv1.3 and hKv1.5 channels with IC50 of 1.3, 1.45 and 1.23 μM in HEK 293 cells.

Cell Assay: In the human leukemia cell lines HL-60 and U937, BAPTA/AM (10 μM) induced internucleosomal DNA cleavage and classic apoptotic morphology. Also, BAPTA/AM increased Ca2+ in intracellular and downregulated c-jun. In bovine chromaffin cells, APTA-AM (50 μM) rapidly and reversibly inhibited Ca2+-activated K+ (I(KCa)) and voltage-gated K+ (I(K)) by 50%. In HEK 293 cells, BAPTA-AM inhibited hERG (Kv11.1), hKv1.3 and hKv1.5 channels with IC50 values of 1.3, 1.45 and 1.23 μM respectively in a concentration dependent way, which was dependent on channel opening.

In VivoBAPTA-AM abolishes the UDCA- and TUDCA-induced increases in Ca2+ levels or Ca2+-Dependent PKC-α phosphorylation, and neutralizes the cytoprotective effects of UDCA and TUDCA on the vagotomy-induced damage of the biliary tree in the BDL rats.
Animal modelBDL rats
Formulation & DosageDissolved in 0.1% DMSO; 6 mg/kg; i.p. injection
References

Prog Neuropsychopharmacol Biol Psychiatry. 2001 Nov;25(8):1641-59; Biochem Pharmacol. 2007 Dec 3;74(11):1596-607; Am J Pathol. 2006 Feb;168(2):398-409.