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Lycopodine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Lycopodine图片
CAS NO:466-61-5
规格:98%
分子量:247.38
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
Lycopodine,一种石松 (Lycopodium clavatumspores) 孢子重要的生物活性成分,通过调节难治性前列腺癌细胞中 5-脂氧合酶 (5-lipoxygenase),极化线粒体膜电位而不调节 p53 活性来诱导细胞凋亡。 Lycopodine 通过激活 caspase-3 诱导细胞凋亡来抑制 HeLa 细胞的增殖。
CAS:466-61-5
分子式:C16H25NO
分子量:247.38
纯度:98%
存储:Store at -20°C

Background:

Lycopodine, a pharmacologically important bioactive component derived from Lycopodium clavatumspores, triggers apoptosis by modulating 5-lipoxygenase, and depolarizing mitochondrial membrane potential in refractory prostate cancer cells without modulating p53 activity[1]. Lycopodine inhibits proliferation of HeLa cells through induction of apoptosis via caspase-3 activation[2]. Caspase-3


Lycopodine (5.22-78.3 μg/mL; 12 hours) has 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively[1].Treated with Lycopodine (74-222 mM; 12 hours), the apoptotic index is with respect to the gradual increase in doses for the PC3 and LnCaP cells[1].Lycopodine (74-222 mM; 12 hours) induces cell cycle arrest at G0/G1 phase in PC3 and LnCaP cells[1].Lycopodine (0-200 µg/mL; 48 hours) shows cytotoxicity to HeLa cells in a dose and time dependent manner. However, Lycopodine shows minimal cytotoxic effects in normal peripheral blood mononuclear cells (PBMC) even at the highest dose (200 µg/mL)[2].Lycopodine (100, 200 µg/mL; 24 hours) increases level of Bax and decreases the mitochondrial cytochrome c. This is followed by an increase in expression of cytochrome c in cytosolic fraction. Lycopodine also cleaves the caspase-3 in the total cell lysate, while the expression of Bcl-2 is down regulated[2]. Cell Viability Assay[1] Cell Line: PC3 and LnCaP cells



[1]. Bishayee K, et al. Lycopodine triggers apoptosis by modulating 5-lipoxygenase, and depolarizing mitochondrial membrane potential in androgen sensitive and refractory prostate cancer cells without modulating p53 activity: signaling cascade and drug-DNA interaction. Eur J Pharmacol. 2013 Jan 5;698(1-3):110-21. [2]. Mandal SK, et al. Lycopodine from Lycopodium clavatum extract inhibits proliferation of HeLa cells through induction of apoptosis via caspase-3 activation. Eur J Pharmacol. 2010 Jan 25;626(2-3):115-22.