Background:

Epothilone B (EPO906, Patupilone) is a naturally occurring microtubule-stabilizing macrolide that was first isolated from the myxobacterium?Sorangium cellulosum. The EC0.01 value of Epothilone B is?1.8 nM [1].

Epothilone B exhibits the same mechanism of action as paclitaxel.?More importantly, in cell culture, Epothilone B is active against paclitaxel-resistant cell lines that express P-glycoprotein. Furthermore, Epothilone B has better solubility in water than paclitaxel [1].

Epothilone B induced an increase in the level of the intracellular free calcium only during the longer times following the treatment in?SKOV-3 cells. Besides, the ovarian cancer cells treated with Epothilone B showed a time course dependent increase in the cytosolic fraction of cytochrome c [2].

参考文献:
[1] Regueiro-Ren A1,?Borzilleri RM,?Zheng X,?Kim SH,?Johnson JA,?Fairchild CR,?Lee FY,?Long BH,?Vite GD. Synthesis?and?biological?activity?of?novel?epothilone?aziridines. Org Lett.?2001 Aug 23;3(17):2693-6.
[2] Rogalska A1,?Gajek A2,?Marczak A2. Epothilone B induces extrinsic pathway of apoptosis in human SKOV-3 ovarian cancer cells. Toxicol In Vitro.?2014 Jun;28(4):675-83.