Background:

Quin-2AM is a fluorecent Ca2+ chelator, with high affinity for calcium.

Quin-2AM (20 µM) or A23187 (20 µM) drastically activate mTF activity without detectable effect on mTF synthesis; both of which show that sustained functional upregulation during 24 h culture does not enhance Marcks expression. Quin-2AM (20 µM) changes the non-detectable Marcks level in 24 h cultures[1].

[1]. Chu AJ, et al. Possible role of Marcks in the cellular modulation of monocytic tissue factor-initiated hypercoagulation. Br J Haematol. 2002 Aug;118(2):569-76.