Background:

Akt functions as a key component in multiple signaling pathways to promote cell survival by mediating resistance to apoptosis. Akt inhibitor X is a cell permeable phenoxazine derivative that suppresses insulin-like growth factor 1 (IGF-1)-stimulated phosphorylation of Akt with an IC50 value of 1-2 μM.[1] This compound has been shown to block IGF-1-stimulated nuclear translocation of Akt in Rh1 cells and to inhibit the growth of Rh1, Rh18, and Rh30 cells with IC50 values of 2-5 μM.[1] Inhibition of Akt phosphorylation by 5 μM of this compound strongly correlates to the inhibition of the downstream targets, mTOR, p70S6 kinase, and S6 ribosomal protein and to an increase in apoptosis.[1]

Reference:
[1]. Thimmaiah, K.N., Easton, J.B., Germain, G.S., et al. Identification of N10-substituted phenoxazines as potent and specific inhibitors of Akt signaling. The Journal of Biological Chemisty 280(36), 31924-31935 (2005).