DMPO is a kind of water-soluble nitric oxide spin trap, which allows the measurement of oxygen-centered free radicals in biological systems at room temperature using electron spin resonance. It has a high reaction rate constant for superoxide and hydroxyl radicals and distinguishes simultaneously among a variety of important biologically generated free radicals.[1][2]

CAS：3317-61-1
分子式：C6H11NO
分子量：113.16
纯度：98%
存储：Store at -20°C

Background:

In vitro, It was demonstrated in red blood cells and in hamster V79 cells that the DMPO spin adducts of ·O2? and ·OH are metabolized very rapidly so that even if formed, they may not be detected in many experiments with cells [1].

In vivo, when DMPO perfused the heart at 100 μm/L, it attenuated the development of reperfusion arrhythmias during the first 10 min of reperfusion and improved the functional recovery of the heart during reperfusion. Without treatment, 55% of hearts showed irreversible ventricular fibrillation, and this was completely prevented by DMPO, due to its function of traping·O2? and ·OH radicals [2].

参考文献：

[1] Samuni A1, Samuni A, Swartz HM. The cellular-induced decay of DMPO spin adducts of ·O2? and ·OH. Free Radic Biol Med. 1989; 6(2):179-83.

[2] Tosaki A1, Blasig IE, Pali T, et al. Heart protection and radical trapping by DMPO during reperfusion in isolated working rat hearts. Free Radic Biol Med. 1990; 8 (4):363-72.