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Ustekinumab
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
CAS NO:943609-66-3
规格:≥98%
包装与价格：
包装价格(元)
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议

产品介绍

    In vitroUstekinumab is a human monoclonal antibody that prevents human IL-12 and IL-23 from binding to the IL-12Rβ1 receptor chain of IL-12 (IL-12Rβ1/β2) and IL-23 (IL-12Rβ1/23R) receptor complexes on the surface of natural killer cells and T-cells[1]. Ustekinumab is a human IgG1 kappa (κ) monoclonal antibody against the IL-12/IL-23 p40 subunit that prevents its interaction with IL-12Rβ1, thereby blocking subsequent signaling. The FcRn affinities at pH 6.0 fell in a narrow range for all 11 antibodies. The equilibrium dissociation constant (KD) is calculated relative to Ustekinumab (Ustekinumab=1.0).
    In VivoN/A
    ProtocolCell Assay: HUVECs are grown on calibrated glass coverslips to a confluency of 80%. Subsequently, cells are incubated with 200 μg/mL Ustekinumab AlexaFluor488 and Briakinumab AlexaFluor594 for 20 min at 37°C, pH 7.3, washed three times, and chased in fresh medium for an additional 20 min before fixation. Antibodies are directly labeled. FcRn binding is quality controlled by SPR analysis. Cells are then counterstained with anti-FcRn monoclonal antibody DVN22, followed by anti-mouse secondary antibodies conjugated to AlexaFluor647. Imaging is performed on a Leica SP8 confocal microscope.
    ReferencesOpen Access Rheumatol. 2014 Feb 20;6:7-13.; Proc Natl Acad Sci U S A. 2015 May 12;112(19):5997-6002.