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Tetrandrine(NSC-77037)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Tetrandrine(NSC-77037)图片
CAS NO:518-34-3
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
500mg电议
1g电议
2g电议
5g电议
10g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)622.75
FormulaC38H42N2O6
CAS No.518-34-3
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: <1 mg/mL
Water: <1 mg/mL
Ethanol: <1 mg/mL
Other infoChemical Name: (11S,31S)-16,36,37,54-tetramethoxy-12,32-dimethyl-11,12,13,14,31,32,33,34-octahydro-2,6-dioxa-1(7,1),3(8,1)-diisoquinolina-5(1,3),7(1,4)-dibenzenacyclooctaphane
SMILES Code: COC1=C(C2=C(C=C1OC)CCN(C)[C@]2(C3)[H])OC4=CC5=C(C=C4OC)CCN(C)[C@]5(CC6=CC=C(OC7=CC3=CC=C7OC)C=C6)[H]
SynonymsNSC 77037; Sinomenine A; d-tetrandrine; Fanchinine; Tetrandrine; NSC-77037; NSC77037; (S,S)-(+)-tetrandrine; TTD.
实验参考方法
In Vitro

In vitro activity: Tetrandrine inhibits voltage-gated Ca2+ currents with an IC50 of 10.1 mM in isolated nerve terminals of the rat neurohypophysis. Tetrandrine is a high-affinity blocker of the type II, maxi-Ca(2+)-activated K+ channel of the rat neurohypophysial terminals with an IC50 of 0.21 mM. Another study shows that Tetrandrine also inhibits Ca(2+)-activated Cl- currents (I(Cl,Ca)) with an IC50 of 5.2 mM. Tetrandrine inhibits the proliferation of human leukemic HL-60 cells via induction of apoptosis.


Cell Assay: Huh7, HCCLM9 and Hep3B cells are seeded in a 96-well plate at a cell density of 5 × 103 cells/well. The cells are treated with the indicated concentrations (0-4 μM) of Tetrandrine (NSC-77037) for 24 h. The cells are subsequently stained with 20 μL of MTS for 1-2 h, and the plates are read at 490 nm on a BioTek ELx800.

In VivoTo evaluate the effect of Tetrandrine (NSC-77037) on the inhibition of tumor metastasis in vivo, HCCLM9 subcutaneous tumor xenograft models is established with athymic nude mice. When the tumor volume reach approximately 50 mm3, nude mice are orally administered vehicle or Tetrandrine (NSC-77037) (30 mg/kg) every other day for 37 days. Tetrandrine (NSC-77037) treatment inhibits tumor growth by reducing the tumor volume and weight.
Animal modelMice
Formulation & DosageFour-week-old male athymic BALB/c nu/nu SPF mice (body weight range from 18 g to 20 g) are used. HCCLM9 WT and HCCLM9 ATG7 KO cells (5 million) resuspended in 0.2 mL of PBS are subcutaneously implanted into the right flank of each mouse. When the tumor volume reach approximately 50 mm3, the tumor-bearing mice are randomly divided into control and treatment groups (n = 6). The control and treatment groups are administered oral injection of vehicle (0.5% methylcellulose) and Tetrandrine at 30 mg/kg of body weight every other day for 37 days. During the treatment, the tumor volumes are measured every day and are calculated.
ReferencesPflugers Arch. 1992 Sep;421(6):558-65; Acta Pharmacol Sin. 2004 Mar;25(3):327-33; J Exp Clin Cancer Res. 2018 Jan 15;37(1):7.