Ellipticine

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Ellipticine

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	519-23-3
包装:	10mg

包装与价格：

包装	价格(元)
10mg	电议

产品名称

CCG36483
NSC71795

产品介绍

Ellipticine是一种DNA拓扑异构酶II抑制剂。Ellipticine玫瑰树碱作用的另一种方式是通过其与细胞色素P450（CYP）和过氧化物酶的氧化介导的共价DNA加合物的形成。Ellipticine还可以作为生物转化酶的抑制剂或诱导剂，从而调节其自身的代谢，从而产生其遗传毒性和药理作用。

体内研究显示，Ellipticine治疗导致在几种健康器官（肝，肾，肺，脾，乳腺，心脏和脑）和乳腺癌DNA中产生玫瑰树碱衍生的DNA加合物。在这些腺癌中产生的玫瑰树碱衍生的DNA加合物的水平几乎是正常健康乳腺组织的2倍。

细胞实验
细胞系	MCF-7, U87MG, CCRF-CEM, UKF-NB-3 and UKF-NB-4 neuroblastoma cell lines
方法	Cells in exponential growth were seeded at 1×10⁴per well in a 96-well microplate. After incubation (48 hours) at 37 °C in 5% CO2 saturated atmosphere the MTT solution (2 mg/ml PBS) was added, the microplates were incubated for 4 hours and cells lysed in 50% N,N-dimethylformamide containing 20% of sodium dodecyl sulfate (SDS), pH 4.5. The absorbance at 570 nm was measured for each well by multiwell ELISA reader Versamax (Molecular devices, CA, USA). The mean absorbance of medium controls was subtracted as a background. The viability of control cells was taken as 100% and the values of treated cells were calculated as a percentage of control. The IC50 values were calculated from at least 3 independent experiments using linear regression of the dose-log response curves by SOFTmaxPro.
浓度	0, 0.1, 1, 5 or 10 μM
处理时间	48 hours

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.0599 mL	20.2996 mL	40.5992 mL
5 mM	0.812 mL	4.0599 mL	8.1198 mL
10 mM	0.406 mL	2.03 mL	4.0599 mL