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MCB-613
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
MCB-613图片
CAS NO:1162656-22-5
规格:≥98%
包装与价格:
包装价格(元)
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议
1g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)304.39
FormulaC20H20N2O
CAS No.1162656-22-5
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 60 mg/mL (197.1 mM)
Water: <1 mg/mL
Ethanol: 60 mg/mL (197.1 mM)
Solubility (In vivo)4% DMSO+30% PEG 300+ddH2O: 2mg/mL
SynonymsMCB-613; MCB 613; MCB613
实验参考方法
In Vitro

In vitro activity: MCB-613 selectively and reversibly binds to the RID of SRC-3, and selectively kills cancer cells including MCF-7 (breast), PC-3 (prostate), H1299 (lung), and HepG2 (liver) cells, without toxicity to mouse primary hepatocytes and mouse embryonic fibroblasts (MEFs). MCB-613 also increases SRCs’ interactions with other coactivators and markedly induces ER stress coupled to the generation of reactive oxygen species (ROS).


Kinase Assay: After various compound treatments, cells are lysed in luciferase lysis buffer and assayed for luciferase activity using the ONE-Glo luciferase assay system. All luciferase activities are normalized to protein concentration determined by Bradford assay.


Cell Assay: Cells [MCF-7 (breast), PC-3 (prostate), H1299 (lung), and HepG2 (liver) cells; mouse primary hepatocytes and mouse embryonic fibroblasts] are seeded in 96-well plates and allowed to reach 60% to 70% confluence. After indicated compound treatments, relative numbers of viable cells are measured by MTS assay using the Cell Titer 96 Aqueous One Solution Cell Proliferation Assay.

In VivoIn an MCF-7 breast cancer mouse xenograft model, MCB-613 (20 mg/kg, i.p.) significantly and dramatically inhibits the growth of the tumor while causing no obvious animal toxicity and body weight less.
Animal modelMice bearing MCF-7 xenografts
Formulation & DosageDissolved in saline;20 mg/kg; i.p. injection
References

Cancer Cell. 2015 Aug 10;28(2):240-52