Combretastatin A4

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Combretastatin A4

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	117048-59-6
包装:	5mg, 10mg

包装与价格：

产品名称

CRC 87-09, NSC 817373, CA-4

产品介绍

Combretastatin A4 (CA-4)是从Combretum caffrum中分离得到的一种天然二苯乙烯，是一种微管稳定剂，可结合β-微管蛋白上的秋水仙碱结构域，比紫杉醇或长春花生物碱具有更低的毒性。

细胞实验
细胞系	MDA-MB-231, A549, and Hela cells
方法	Growing MDA-MB-231, A549, and HeLa cells in DMEM medium (115 units/mL of penicillin G, 115 μg/mL of streptomycin, and 10% fetal bovine serum). Seeding cells in 96-well plates (5000 cells/well) containing 50 μL of growth medium for 24 h. After medium removal, adding 100 μL of fresh medium containing individual analogue compounds at different concentrations to each well and incubating at 37 ℃ for 72 h. After 24 h of culture, supplementing the cells with 50 μL of analogue compounds dissolved in DMSO (less than 0.25% in each preparation). After 72 h of incubation, adding 20 μL of resazurin for 2 h before recording fluorescence at 560 nm (excitation) and 590 nm (emission) using a Victor microtiter plate fluorimeter. Defining the IC50 as the compound concentration required to inhibit cell proliferation by 50% in comparison with cells treated with the maximum amount of DMSO (0.25%) and considered as 100% viability.
浓度	~3.8 nM
处理时间	72 h

动物实验
动物模型	FVB/N or nude NMRI female mice bearing NT2 and MDA-MB-231 tumors
配制	DMSO
剂量	100 mg/kg
给药处理	i.p.

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.1611 mL	15.8053 mL	31.6106 mL
5 mM	0.6322 mL	3.1611 mL	6.3221 mL
10 mM	0.3161 mL	1.5805 mL	3.1611 mL