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DMOG(dimethyloxaloylglycine)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
DMOG(dimethyloxaloylglycine)图片
CAS NO:89464-63-1
规格:≥98%
包装与价格:
包装价格(元)
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议
1g电议
2g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)175.14
FormulaC6H9NO5
CAS No.89464-63-1
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 35 mg/mL (199.8 mM)
Water: 35 mg/mL (199.8 mM)
Ethanol: 35 mg/mL (199.8 mM)
SMILES Code O=C(OC)CNC(C(OC)=O)=O
SynonymsDimethyloxalylglycine
实验参考方法
In Vitro

In vitro activity: DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC.


Kinase Assay:


Cell Assay: To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at –70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

In VivoDMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats.
Animal modelC57Bl6 Mice
Formulation & Dosage8 mg DMOG dissolved in 0.5 ml saline; 8 mg/mouse; i.p. injection
References

Biochem J. 1994 Jun 1;300 ( Pt 2):525-30; J Physiol. 2004 Oct 1;560(Pt 1):21-6; Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):112-8.