Anemarsaponin B is a naturally occuring steroidal saponin extracted from the rhizomes of A. asphodeloides (Liliaceae). It has anti-inflammatory effect in LPS-treated RAW 264.7macrophages, the
effect is associated with the inhibition of NF-κB transcriptional
activity, possibly via the p38 MAP kinase pathway. Anemarsaponin B can
inhibit PAF-induced rabbit platelet aggregation in vitro. Anemarsaponin B decreases the protein and mRNA levels of iNOS and COX-2. Anemarsaponin B reduces the expressions and productions of pro-inflammatory cytokines, including TNF-a and IL-6. Anemarsaponin B inhibits the nuclear translocation of the p65 subunit of NF-κB by blocking the phosphorylation of IκBα. Anemarsaponin B also inhibits the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3). Anti-inflammatory effect .