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Anacardic Acid(Hydroginkgolic acid)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Anacardic Acid(Hydroginkgolic acid)图片
CAS NO:16611-84-0
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)348.52
FormulaC22H36O3
CAS No.16611-84-0
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 69 mg/mL (198 mM)
Water: <1 mg/mL
Ethanol: N/A
Other info

Chemical Name: 2-Hydroxy-6-pentadecyl-benzoic acid

InChi Key: ADFWQBGTDJIESE-UHFFFAOYSA-N

InChi Code: InChI=1S/C22H36O3/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-16-19-17-15-18-20(23)21(19)22(24)25/h15,17-18,23H,2-14,16H2,1H3,(H,24,25)

SMILES Code: O=C(O)C1=C(CCCCCCCCCCCCCCC)C=CC=C1O

Synonyms 6-pentadecylsalicylic Acid
实验参考方法
In Vitro

In vitro activity: Anacardic acid is found to be a common inhibitor of several clinically targeted enzymes such as NFkB kinase, histone acetyltransferase (HATs), lipoxygenase (LOX-1), xanthine oxidase, tyrosinase and ureases. Anacardic acid is also an efficient inhibitor of Tip60 in vitro.It inhibits the Tip60-dependent acetylation and activation of the ATM protein kinase in HeLa cells, and sensitizes the cells to the cytotoxic effects of radiation. Anacardic acid can effectively prevent cell damage induced by H2O2 because this can be converted to ROS, hydroxyl radicals, in the presence of metal ions


Kinase Assay: After homogenization of cardiac tissues, nucleoproteins are extracted using a Nuclear Extract Kit according to the manufacturer's instructions. HAT and HDAC activities of the nuclear protein extracts are determined using a colorimetric assay included in the HAT and HDAC assay kits.


Cell Assay: HeLa and 293T cells are grown in Dulbecco’s modified Eagle’s medium/10% fetal calf serum. SQ20B and SCC35 squamous cell carcinoma cell lines are cultured in modified eagles medium/10% fetal calf serum.For anacardic acid (EMD Biosciences, CA) exposure, cells are preincubated with anacardic acid for 40 min, irradiated, then allowed to recover for 4 h. Cells are then switched to fresh media and allowed to grow for 10 days.

In VivoAnacardic acid attenuates over-expression of Gata4, α-MHC and cTnT in fetal mouse hearts exposed to ethanol. Anacardic acid inhibits binding of P300, PCAF to the promoter of Gata4
Animal model Kunming mice
Formulation & Dosage Dissolved in DMSO and co-injected with ethanol; 0, 1.25, 2.5, 5, 10 mg/kg; i.p. injection
References

J Biol Chem. 2003 May 23;278(21):19134-40; FEBS Lett. 2006 Aug 7;580(18):4353-6; PLoS One. 2014 Aug 7;9(8):e104135.