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740 Y-P(PDGFR 740Y-P)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
740 Y-P(PDGFR 740Y-P)图片
CAS NO:1236188-16-1
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品介绍
理化性质和储存条件

Molecular Weight (MW)

3270.70

Formula

C141H222N43O39PS3

CAS No.

1236188-16-1

Storage

-20℃ for 3 years in powder form

-80℃ for 2 years in solvent

Solubility (In vitro)

DMSO: N/A

Water: 20 mg/mL

Ethanol: N/A

Other info

Sequence: Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-{PO2-Tyr}-Met-Asp-Met-Ser
Sequence Shortening: R-Q-I-K-I-W-F-Q-N-R-R-M-K-W-K-K-S-D-G-G-{PO2-Y}-M-D-M-S
SMILES: [RQIKIWFQNRRMKWKKSDGG-{PO2-Tyr}-MDMS]

Synonyms

740 Y-P; 740 YP; 740YPDGFR; PDGFR740Y-P; 740 Y P; 740-YPDGFR; PDGFR 740 Y-P; 740 YPDGFR; PDGFR 740Y-P

实验参考方法

In Vitro

In vitro activity: The PDGFR 740Y-P peptide stimulates a mitogenic response in muscle cells. The ability of the 740Y-P peptide to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides. 740Y-P is as effective as a growth factor (FGF2) at promoting neuronal cell survival via the established PI 3-kinase-Akt survival cascade. Stimulation of neuronal cell survival by the PDGFR740Y-P peptide is dose dependent and does not require insulin.

Kinase Assay: 740 Y-P is an activator of PI3K with concentration of 20 μM.

Cell Assay: NIH 3T3 cells (2×106) are incubated in suspension at 37°C in Ca2+ and Mn2+ free HBSS, 10% FCS containing either 50 μg/ml of 740Y-P peptide or an equal volume of PBS as a control. After 2h cells are centrifuged, washed and trypsinised to degrade non-internalised peptide. Cells are then resuspended in lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 10% Glycerol, 2% NP40, 0.25% deoxycholate, 1 mM EDTA, 1 mM Vanadate, Protease inhibitors 'complete' cocktail from Boehringer-Mannheim) for 1 h at 4°C. Lysates are clarified by centrifugation at 1.4×104g for 5 min and the supernatants are incubated for 1h with streptavidin-agarose beads. Beads are subsequently washed three times in lysis buffer and finally boiled in SDS sample buffer and resolved by SDS-PAGE on a 12% gel, transferred to nitrocellulose and immunoblotted with the p85 monoclonal antibody.

In Vivo

740 Y-P can not only be internalized in living cells but can also interact with p85 in vivo.

Animal model

N/A

Formulation & Dosage

N/A

References

Mol Cell Neurosci. 1999 Apr;13(4):272-80; Biochem Biophys Res Commun. 1998 Oct 9;251(1):148-52; PLoS One. 2014 Aug 29;9(8):e105965.