CAS NO: | 1236188-16-1 |
规格: | ≥98% |
包装 | 价格(元) |
5mg | 电议 |
10mg | 电议 |
25mg | 电议 |
50mg | 电议 |
100mg | 电议 |
Molecular Weight (MW) | 3270.70 |
Formula | C141H222N43O39PS3 |
CAS No. | 1236188-16-1 |
Storage | -20℃ for 3 years in powder form |
-80℃ for 2 years in solvent | |
Solubility (In vitro) | DMSO: N/A |
Water: 20 mg/mL | |
Ethanol: N/A | |
Other info | Sequence: Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-{PO2-Tyr}-Met-Asp-Met-Ser |
Synonyms | 740 Y-P; 740 YP; 740YPDGFR; PDGFR740Y-P; 740 Y P; 740-YPDGFR; PDGFR 740 Y-P; 740 YPDGFR; PDGFR 740Y-P |
In Vitro | In vitro activity: The PDGFR 740Y-P peptide stimulates a mitogenic response in muscle cells. The ability of the 740Y-P peptide to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides. 740Y-P is as effective as a growth factor (FGF2) at promoting neuronal cell survival via the established PI 3-kinase-Akt survival cascade. Stimulation of neuronal cell survival by the PDGFR740Y-P peptide is dose dependent and does not require insulin.
Kinase Assay: 740 Y-P is an activator of PI3K with concentration of 20 μM.
Cell Assay: NIH 3T3 cells (2×106) are incubated in suspension at 37°C in Ca2+ and Mn2+ free HBSS, 10% FCS containing either 50 μg/ml of 740Y-P peptide or an equal volume of PBS as a control. After 2h cells are centrifuged, washed and trypsinised to degrade non-internalised peptide. Cells are then resuspended in lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 10% Glycerol, 2% NP40, 0.25% deoxycholate, 1 mM EDTA, 1 mM Vanadate, Protease inhibitors 'complete' cocktail from Boehringer-Mannheim) for 1 h at 4°C. Lysates are clarified by centrifugation at 1.4×104g for 5 min and the supernatants are incubated for 1h with streptavidin-agarose beads. Beads are subsequently washed three times in lysis buffer and finally boiled in SDS sample buffer and resolved by SDS-PAGE on a 12% gel, transferred to nitrocellulose and immunoblotted with the p85 monoclonal antibody. |
In Vivo | 740 Y-P can not only be internalized in living cells but can also interact with p85 in vivo. |
Animal model | N/A |
Formulation & Dosage | N/A |
References | Mol Cell Neurosci. 1999 Apr;13(4):272-80; Biochem Biophys Res Commun. 1998 Oct 9;251(1):148-52; PLoS One. 2014 Aug 29;9(8):e105965. |