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PRT4165(NSC-600157)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
PRT4165(NSC-600157)图片
CAS NO:31083-55-3
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议
1g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW) 235.24
Formula C15H9NO2
CAS No. 31083-55-3
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 11 mg/mL (46.8 mM)
Water: 4 mg/mL (17.0 mM)
Ethanol: < 1 mg/mL
Chemical Name 2-(3-Pyridinylmethylene)-1H-Indene-1,3(2H)-dione
Synonyms PRT4165, PRT-4165, PRT 4165; NSC600157; NSC 600157; NSC-600157
SMILES Code O=C1/C(C(C2=C1C=CC=C2)=O)=C\C3=CC=CN=C3
实验参考方法
In Vitro

In vitro activity: In vitro E3 ubiquitin ligase activity assays revealed that PRT4165 inhibits both RNF2 and RING 1A, which are partially redundant paralogues that together account for the E3 ubiquitin ligase activity found in PRC1 complexes, but not RNF8 nor RNF168.


Cell Assay: Cells are treated with different concentrations of the PRT4165 60 min prior to irradiation (2 Gy). The cells are harvested 2 h after IR. The cells are washed with PBS twice and then fixed with 1% paraformaldehyde at 37°C for 10 min. After cooling on ice for 1 min, the cells are permeabilized with 90% methanol and stored at -20°C overnight. Fixed cells are washed with PBS twice and blocked with FACS incubation buffer (0.5% BSA in PBS) for 10 min. The cells are then stained with anti-phosphohistone H3 (serine 10) antibody at 1:500 dilution in FACS incubation buffer for 1 h at room temperature.

In VivoInhibition of PRC1 causes alteration of gene regulation and polyploidy development during decidualization in vivo.
Animal model CD-1 mice
Formulation & Dosage Dissolved in sesame oil containing 10% ethanol; 4 mg/kg and 8 mg/kg; intraluminally injection
References J Biol Chem. 2013 Sep 13;288(37):26944-54; Sci Rep. 2016; 6: 26061.