2H-Cho-Arg is a steroid-based cationic lipid that contains a 2H-cholesterol skeleton coupled to an L-arginine head group and can be used to facilitate gene transfection. It forms a complex with plasmid DNA (pDNA) and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher. 2H-Cho-Arg facilitates transfection of a luciferase gene into H1299 cells, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin and the caveolae-mediated endocytosis inhibitor genistein , but not by inhibitors of clathrin- or micropinocytosis-mediated endocytosis. It induces cytotoxicity in H1299 cells (IC50 = 92.7 μg/ml).

1609010-59-4

C43H73F6N5O7

886.06

2H-Cho-Arg (trifluoroacetate salt)

Ethanol:50 mg/mL
DMSO:50 mg/mL
DMF:50 mg/mL
Ethanol:PBS (pH 7.2) (1:3):0.25 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years