Thioester analogs of glycerophospholipids, in combination with Ellman's reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity. Palmitoyl thio-PC is a chromogenic PLA2 substrate that contains a palmitoyl thioester at the sn-2 position of the glycerol backbone. Hydrolysis of the thioester by PLA2 yields a free thiol that reacts readily with DTNB (Ellman's reagent) giving a bright yellow product with an absorbance maximum at 412 nm. Palmitoyl thio-PC has been used to measure bee venom sPLA2 activity in a phospholipid:Triton X-100 mixed micelle system.

113881-60-0

C40H80NO7PS

750.1

Palmitoyl thio-PC

Ethanol:50 mg/mL
Ethanol:PBS (pH 7.2) (1:1):0.5 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years