7α-Hydroxy-4-cholesten-3-one is an intermediate in the synthesis of bile acids from cholesterol. It is a PXR agonist. 7α-Hydroxy-cholest-4-en-3-one is a biomarker for bile acid loss and other diseases associated with defective bile acid biosynthesis.It is the physiological substrate for CYP8B1.

7α-Hydroxy-4-cholesten-3-one is found relatively upstream in the biosynthetic pathway to bile acids. The first step is the incorporation of the 7α-hydroxy group onto cholesterol by cytochrome P450 7A1, and the second step is the oxidation and isomerization of the 3-hydroxy group and the Δ5,6-double bond by a 3β-hydroxysteroid dehydrogenase to yield 7α-Hydroxy-4-cholesten-3-one. The deletion of the gene that expresses P450 27A1, which is found downstream in the bile acid pathway, results in the accumulation of the precursor, 7α-Hydroxy-4-cholesten-3-one [1].

7α-Hydroxy-4-cholesten-3-one strongly relates to the hepatic enzymatic activity of CYP7A1 at steady-state conditions as well as during the rapid diurnal changes that occur in the rat. That serum 7α-Hydroxy-4-cholesten-3-one has a pronounced diurnal rhythm [2].

3862-25-7

C27H44O2

400.64

7α-Hydroxy-4-cholesten-3-one

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years