7,12-Dimethylbenz[a]anthracene (DMBA) is a polycyclic aromatic hydrocarbon (PAH) that has been found in tobacco smoke and diesel exhaust and has carcinogenic activity.1,2It undergoes metabolic activation by numerous enzymes, including the cytochrome P450 (CYP450) isoform CYP1B1, as well as microsomal epoxide hydrolase (mEH), producing a variety of reactive metabolites that form DNA adductsin vivo, and it has been commonly used to induce tumor formation in various rodent models.2,3,1DMBA increases proliferation and migration of, and induces epithelial-to-mesenchymal transition (EMT) in, MCF-10A breast cancer cells when used at a concentration of 5 μM.4It also has immunosuppressant activity, inhibiting proliferation of isolated mouse splenic B- and T cells stimulatedex vivowith LPS or concanavalin A , respectively, when administered at doses of 50 and 100 mg/kg.5Intragastric administration of DMBA (20 mg/animal) induces formation of mammary gland tumors in rats.6DMBA (50 μg/animal), in combination with phorbol 12-myristate 13-acetate , initiates papilloma formation in a rat two-stage model of skin carcinogenesis.7

57-97-6

C20H16

256.34

DMBA

DMSO:10 mg/mL
DMF:20 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years