5-FAM 是一种绿色荧光探针(Ex/Em 为 490 nm/520 nm)，可用于肽，蛋白质和核苷酸的标记。

5-FAM (5-Carboxyfluorescein) contains a carboxylic acid, which can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its excellent fluorescence quantum yield, relatively high absorptivity, and good water solubility, it also has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

76823-03-5

C21H12O7

376.32

5-羧基荧光素;5-Carboxyfluorescein;5-FAM

DMSO:30 mg/mL (79.72 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years