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Sulfo-NHS-SS-Biotin sodium
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Sulfo-NHS-SS-Biotin sodium图片
CAS NO:325143-98-4
包装与价格:
包装价格(元)
10 mg电议
50 mg电议
100 mg电议

产品名称
Sulfo-NHS-SS-Biotin
Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate
Sulfo-NThiol-SS-biotin
Sulfo-NHS-SS-Biotin (sodium)
产品介绍
Sulfo-NHS-SS-Biotin sodium 是一种长链可裂解和细胞无渗透的胺类活性生物素化 (biotinylation) 试剂。Sulfo-NHS-SS-Biotin sodium 可用于细胞表面蛋白 (cell-surface protein) 的标记和纯化。

产品描述

Sulfo-NHS-SS-Biotin sodium is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein.

体外活性

Sulfo-NHS-SS-biotin is a negatively charged reagent and does not permeate cell membranes. Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides[1]. Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail [2].

Cas No.

325143-98-4

分子式

C19H28N4NaO9S4

分子量

607.7

别名

Sulfo-NHS-SS-Biotin;Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate;Sulfo-NThiol-SS-biotin;Sulfo-NHS-SS-Biotin (sodium);Sulfo-NHS-SS-Biotin sodium

储存和溶解度

DMSO:100 mg/mL (164.8 mM),Need ultrasonic
H2O:180 mg/mL (296.7 mM),Need ultrasonic
Powder: -20°C for 3 years
In solvent: -80°C for 2 years